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fitc anti sheep cd45  (Bio-Rad)


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    Bio-Rad fitc anti sheep cd45
    Fitc Anti Sheep Cd45, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+anti+sheep+cd45/Mouse+anti+Sheep+CD45/10__1016_slash_j__foodres__2026__118616-61-65-69
    Average 94 stars, based on 91 article reviews
    fitc anti sheep cd45 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    FACS:

    Article Title: Machine learning framework for passage-based quality control of Hanwoo muscle satellite cells for cultured meat
    Article Snippet: Myogenic satellite cells from Hanwoo (Korean native cattle) are valuable for cultured meat and livestock biotechnology, serving as a primary cellular substrate for the biomanufacturing of alternative protein, particularly cultured beef, and enabling the development of standardized, high-value cultured beef products.. We established a multivariate grading framework that integrates indicators of proliferation and senescence to objectively assess passage-dependent cellular quality.. Missing biomarker values (G0/G1 phase, early apoptosis, γH2AX, SA-β-galactosidase, telomerase) were imputed using live cell number, with random forest regression showing superior performance (R2 > 0.93).

    Article Title: Optimizing muscle satellite cell sources for cultured meat: anatomical origin influences cellular properties and quality attributes
    Article Snippet: The cells were cultured for 72 h and then detached using trypsin-EDTA (Gibco) to purify the PMSCs. .. The cells were reconstituted using fluorescence-activated cell sorting (FACS) solution (1% bovine serum albumin [BSA] in PBS) and stained with APC anti-human CD29 antibody (1:20; BioLegend, USA), PE-Cy TM7 anti-human CD56 antibody (1:20; BD Biosciences, USA), FITC anti-sheep CD31 (1:20; Bio-Rad, USA), and FITC anti-sheep CD45 (1:20; Bio-Rad) for 30 min on ice. ..

    Article Title: Characteristics of bovine muscle satellite cell from different breeds for efficient production of cultured meat
    Article Snippet: Cells for FACS were grown in a humidified incubator (5% CO 2 , 37°C) for 5 days on bovine collagen type I (A1064401, Gibco) coated flasks. .. Cells were suspended in FACS buffer (1:100 bovine serum albumin [BSA] in phosphate buffered saline [PBS]) and stained with APC anti-human CD29 antibody (303008, BioLegend, San Diego, CA, USA), PE-CyTM7 anti-human CD56 (335826, BD, Franklin Lakes, NJ, USA), FITC anti-sheep CD31 (MCA1097GA, Bio-Rad, Hercules, CA, USA), and FITC anti-sheep CD45 (MCA2220GA, Bio-Rad). .. After antibody incubation, cells were washed multiple times with 1X PBS and repaced to Ham’s F-10 nutrient mix (11550043, Gibco) with 1% PSA antibiotics and 20% fetal bovine serum (FBS; 2335015CV, Corning).

    Article Title: Drone pupae extract enhances Hanwoo myosatellite cell function for cultivated meat production
    Article Snippet: Harvested HSC were stored in liquid nitrogen using a freezing medium (Cell Culture Freezing Medium, Cat # 12648-010, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and considered as passage 0. .. Some HSC were cultured and then analyzed by FACS with the following antibodies: APC anti-human CD29 antibody (1:10, Cat # 303008, BioLegend, San Diego, CA, USA), PE-CyTM7 anti-human CD56 (1:10, Cat # 335826, BD, Franklin Lakes, NJ, USA), FITC anti-sheep CD31 (1:10, Cat # MCA1097GA, Bio-Rad Laboratories, Hercules, CA, USA), and FITC anti-sheep CD45 (1:10, MCA2220GA, Bio-Rad). .. These HSC were sorted into CD31-/CD45-, CD29+/CD56+ populations using a FACS Aria II Cell Sorter (BD Life Sciences, San Jose, CA, USA).

    Article Title: Drone pupae extract enhances Hanwoo myosatellite cell function for cultivated meat production.
    Article Snippet: Harvested HSC were stored in liquid nitrogen 164 using a freezing medium (Cell Culture Freezing Medium, Gibco, Cat # 12648-010) and considered as passage 0. .. 165 AC CE PT ED Some HSC were cultured and then analyzed by FACS with the following antibodies: APC anti-human CD29 166 antibody (1:10, BioLegend, USA, Cat # 303008), PE-CyTM7 anti-human CD56 (1:10, BD, USA, Cat # 335826), 167 FITC anti-sheep CD31 (1:10, Bio-Rad, USA, Cat # MCA1097GA), and FITC anti-sheep CD45 (1:10, 168 MCA2220GA, Bio-Rad, USA). .. These HSC were sorted into CD31-/CD45-, CD29+/CD56+ populations using a 169 FACS Aria II Cell Sorter (BD Life Sciences, San Jose, CA, USA).

    Article Title: Optimizing muscle satellite cell sources for cultured meat: anatomical origin influences cellular properties and quality attributes.
    Article Snippet: The cells were cultured for 72 h and then detached using trypsin-EDTA (Gibco) to purify the PMSCs. .. The cells were reconstituted using fluorescence activated cell sorting (FACS) solution (1% bovine serum albumin [BSA] in PBS) and stained with APC anti-human CD29 antibody (1:20; BioLegend, USA), PECyTM7 anti-human CD56 antibody (1:20; BD Biosciences, USA), FITC anti-sheep CD31 (1:20; Bio-Rad, USA), and FITC anti-sheep CD45 (1:20; Bio-Rad) for 30 min on ice. ..

    Isolation:

    Article Title: Machine learning framework for passage-based quality control of Hanwoo muscle satellite cells for cultured meat
    Article Snippet: Myogenic satellite cells from Hanwoo (Korean native cattle) are valuable for cultured meat and livestock biotechnology, serving as a primary cellular substrate for the biomanufacturing of alternative protein, particularly cultured beef, and enabling the development of standardized, high-value cultured beef products.. We established a multivariate grading framework that integrates indicators of proliferation and senescence to objectively assess passage-dependent cellular quality.. Missing biomarker values (G0/G1 phase, early apoptosis, γH2AX, SA-β-galactosidase, telomerase) were imputed using live cell number, with random forest regression showing superior performance (R2 > 0.93).

    Cell Culture:

    Article Title: Machine learning framework for passage-based quality control of Hanwoo muscle satellite cells for cultured meat
    Article Snippet: Myogenic satellite cells from Hanwoo (Korean native cattle) are valuable for cultured meat and livestock biotechnology, serving as a primary cellular substrate for the biomanufacturing of alternative protein, particularly cultured beef, and enabling the development of standardized, high-value cultured beef products.. We established a multivariate grading framework that integrates indicators of proliferation and senescence to objectively assess passage-dependent cellular quality.. Missing biomarker values (G0/G1 phase, early apoptosis, γH2AX, SA-β-galactosidase, telomerase) were imputed using live cell number, with random forest regression showing superior performance (R2 > 0.93).

    Article Title: Drone pupae extract enhances Hanwoo myosatellite cell function for cultivated meat production
    Article Snippet: Harvested HSC were stored in liquid nitrogen using a freezing medium (Cell Culture Freezing Medium, Cat # 12648-010, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and considered as passage 0. .. Some HSC were cultured and then analyzed by FACS with the following antibodies: APC anti-human CD29 antibody (1:10, Cat # 303008, BioLegend, San Diego, CA, USA), PE-CyTM7 anti-human CD56 (1:10, Cat # 335826, BD, Franklin Lakes, NJ, USA), FITC anti-sheep CD31 (1:10, Cat # MCA1097GA, Bio-Rad Laboratories, Hercules, CA, USA), and FITC anti-sheep CD45 (1:10, MCA2220GA, Bio-Rad). .. These HSC were sorted into CD31-/CD45-, CD29+/CD56+ populations using a FACS Aria II Cell Sorter (BD Life Sciences, San Jose, CA, USA).

    Article Title: Drone pupae extract enhances Hanwoo myosatellite cell function for cultivated meat production.
    Article Snippet: Harvested HSC were stored in liquid nitrogen 164 using a freezing medium (Cell Culture Freezing Medium, Gibco, Cat # 12648-010) and considered as passage 0. .. 165 AC CE PT ED Some HSC were cultured and then analyzed by FACS with the following antibodies: APC anti-human CD29 166 antibody (1:10, BioLegend, USA, Cat # 303008), PE-CyTM7 anti-human CD56 (1:10, BD, USA, Cat # 335826), 167 FITC anti-sheep CD31 (1:10, Bio-Rad, USA, Cat # MCA1097GA), and FITC anti-sheep CD45 (1:10, 168 MCA2220GA, Bio-Rad, USA). .. These HSC were sorted into CD31-/CD45-, CD29+/CD56+ populations using a 169 FACS Aria II Cell Sorter (BD Life Sciences, San Jose, CA, USA).

    Saline:

    Article Title: Machine learning framework for passage-based quality control of Hanwoo muscle satellite cells for cultured meat
    Article Snippet: Myogenic satellite cells from Hanwoo (Korean native cattle) are valuable for cultured meat and livestock biotechnology, serving as a primary cellular substrate for the biomanufacturing of alternative protein, particularly cultured beef, and enabling the development of standardized, high-value cultured beef products.. We established a multivariate grading framework that integrates indicators of proliferation and senescence to objectively assess passage-dependent cellular quality.. Missing biomarker values (G0/G1 phase, early apoptosis, γH2AX, SA-β-galactosidase, telomerase) were imputed using live cell number, with random forest regression showing superior performance (R2 > 0.93).

    Article Title: Characteristics of bovine muscle satellite cell from different breeds for efficient production of cultured meat
    Article Snippet: Cells for FACS were grown in a humidified incubator (5% CO 2 , 37°C) for 5 days on bovine collagen type I (A1064401, Gibco) coated flasks. .. Cells were suspended in FACS buffer (1:100 bovine serum albumin [BSA] in phosphate buffered saline [PBS]) and stained with APC anti-human CD29 antibody (303008, BioLegend, San Diego, CA, USA), PE-CyTM7 anti-human CD56 (335826, BD, Franklin Lakes, NJ, USA), FITC anti-sheep CD31 (MCA1097GA, Bio-Rad, Hercules, CA, USA), and FITC anti-sheep CD45 (MCA2220GA, Bio-Rad). .. After antibody incubation, cells were washed multiple times with 1X PBS and repaced to Ham’s F-10 nutrient mix (11550043, Gibco) with 1% PSA antibiotics and 20% fetal bovine serum (FBS; 2335015CV, Corning).

    Staining:

    Article Title: Machine learning framework for passage-based quality control of Hanwoo muscle satellite cells for cultured meat
    Article Snippet: Myogenic satellite cells from Hanwoo (Korean native cattle) are valuable for cultured meat and livestock biotechnology, serving as a primary cellular substrate for the biomanufacturing of alternative protein, particularly cultured beef, and enabling the development of standardized, high-value cultured beef products.. We established a multivariate grading framework that integrates indicators of proliferation and senescence to objectively assess passage-dependent cellular quality.. Missing biomarker values (G0/G1 phase, early apoptosis, γH2AX, SA-β-galactosidase, telomerase) were imputed using live cell number, with random forest regression showing superior performance (R2 > 0.93).

    Article Title: Optimizing muscle satellite cell sources for cultured meat: anatomical origin influences cellular properties and quality attributes
    Article Snippet: The cells were cultured for 72 h and then detached using trypsin-EDTA (Gibco) to purify the PMSCs. .. The cells were reconstituted using fluorescence-activated cell sorting (FACS) solution (1% bovine serum albumin [BSA] in PBS) and stained with APC anti-human CD29 antibody (1:20; BioLegend, USA), PE-Cy TM7 anti-human CD56 antibody (1:20; BD Biosciences, USA), FITC anti-sheep CD31 (1:20; Bio-Rad, USA), and FITC anti-sheep CD45 (1:20; Bio-Rad) for 30 min on ice. ..

    Article Title: Characteristics of bovine muscle satellite cell from different breeds for efficient production of cultured meat
    Article Snippet: Cells for FACS were grown in a humidified incubator (5% CO 2 , 37°C) for 5 days on bovine collagen type I (A1064401, Gibco) coated flasks. .. Cells were suspended in FACS buffer (1:100 bovine serum albumin [BSA] in phosphate buffered saline [PBS]) and stained with APC anti-human CD29 antibody (303008, BioLegend, San Diego, CA, USA), PE-CyTM7 anti-human CD56 (335826, BD, Franklin Lakes, NJ, USA), FITC anti-sheep CD31 (MCA1097GA, Bio-Rad, Hercules, CA, USA), and FITC anti-sheep CD45 (MCA2220GA, Bio-Rad). .. After antibody incubation, cells were washed multiple times with 1X PBS and repaced to Ham’s F-10 nutrient mix (11550043, Gibco) with 1% PSA antibiotics and 20% fetal bovine serum (FBS; 2335015CV, Corning).

    Article Title: Optimizing muscle satellite cell sources for cultured meat: anatomical origin influences cellular properties and quality attributes.
    Article Snippet: The cells were cultured for 72 h and then detached using trypsin-EDTA (Gibco) to purify the PMSCs. .. The cells were reconstituted using fluorescence activated cell sorting (FACS) solution (1% bovine serum albumin [BSA] in PBS) and stained with APC anti-human CD29 antibody (1:20; BioLegend, USA), PECyTM7 anti-human CD56 antibody (1:20; BD Biosciences, USA), FITC anti-sheep CD31 (1:20; Bio-Rad, USA), and FITC anti-sheep CD45 (1:20; Bio-Rad) for 30 min on ice. ..

    Fluorescence:

    Article Title: Optimizing muscle satellite cell sources for cultured meat: anatomical origin influences cellular properties and quality attributes
    Article Snippet: The cells were cultured for 72 h and then detached using trypsin-EDTA (Gibco) to purify the PMSCs. .. The cells were reconstituted using fluorescence-activated cell sorting (FACS) solution (1% bovine serum albumin [BSA] in PBS) and stained with APC anti-human CD29 antibody (1:20; BioLegend, USA), PE-Cy TM7 anti-human CD56 antibody (1:20; BD Biosciences, USA), FITC anti-sheep CD31 (1:20; Bio-Rad, USA), and FITC anti-sheep CD45 (1:20; Bio-Rad) for 30 min on ice. ..

    Article Title: Optimizing muscle satellite cell sources for cultured meat: anatomical origin influences cellular properties and quality attributes.
    Article Snippet: The cells were cultured for 72 h and then detached using trypsin-EDTA (Gibco) to purify the PMSCs. .. The cells were reconstituted using fluorescence activated cell sorting (FACS) solution (1% bovine serum albumin [BSA] in PBS) and stained with APC anti-human CD29 antibody (1:20; BioLegend, USA), PECyTM7 anti-human CD56 antibody (1:20; BD Biosciences, USA), FITC anti-sheep CD31 (1:20; Bio-Rad, USA), and FITC anti-sheep CD45 (1:20; Bio-Rad) for 30 min on ice. ..

    Blocking Assay:

    Article Title: Grifola frondosa extract as a fetal bovine serum supplement for the culture of bovine muscle satellite cells under low serum conditions
    Article Snippet: .. FITC anti-sheep CD45, FITC anti-sheep CD31, polyvinylidene fluoride (PVDF) membranes, and blocking grade buffer were purchased from Bio-Rad (Hercules, California, USA). .. The APC anti-human CD29 antibody was obtained from BioLegend (San Diego, CA, USA).



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